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Starting in 2012 zooplankton sampling at Green Lake 4 was included in the long term monitoring data set at Niwot Ridge. Immediately after the ice has completely melted from the lakes, zooplankton samples are taken once a week for six consecutive weeks at the deepest portion of the lake from an inflatable raft. Zooplankton were sampled at the deepest location of each lake by pulling a conical net (Wisconsin net) vertically through the water column (i.e., vertical tow sample). For each zooplankton sample obtained, adult organisms were identified to species, or lowest taxonomic level (Chydoridae sp. and Bosminidae sp.). Larvae of cladocerans were counted together as neonates; calanoid and cyclopoid copepodites were counted together as nauplii. Individual body lengths of the first 50 -100 (when possible) individuals of each taxon were recorded using a calibrated eyepiece micrometer and means reported.more » « less
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The overarching goal of this project was to develop a process-based understanding of how watershed-to-lake connections drive nearshore productivity dynamics in a large oligotrophic mountain lake (Lake Tahoe). We addressed this goal through a combined approach of high-frequency sensor deployment and maintenance, ecosystem metabolism modeling, laboratory incubations, and routine monitoring of water chemistry and other parameters. The data we collected as part of this project and the ecosystem metabolism estimates we generated demonstrate how variable ecosystem productivity is in time and space in the nearshore of Lake Tahoe. Although maintenance of the sensor arrays during the exceptional winter of 2023 was challenging, we were able to capture the data necessary to estimate a complete time series of metabolic activity across two years with very different hydroclimatic conditions. Throughout this project we accomplished the following: 1. We generated over two years of daily estimates of ecosystem metabolism (gross primary productivity, ecosystem respiration, and net ecosystem productivity) from multiple locations on both the east and west shores of the lake and from areas in close proximity to and far away from stream water inflows. 2. We measured ammonium (NH4+) and nitrate (NO3-) concentrations in surface water samples from both Glenbrook and Blackwood creeks and the nearshore of Lake Tahoe for over two years. 3. We quantified rates of NH4+ and NO3- uptake in benthic samples of the dominant substrate type collected during peak streamflow, the receding limb, and baseflow conditions in 2023 from multiple locations in the nearshore using established laboratory incubation methods. 4. Finally, we used a combination of time series models and structural equation modeling to integrate our results and improve understanding of the direct and indirect effects of hydroclimatic variability on observed patterns in ecosystem metabolism in the nearshore. See this git code repository for project analysis: https://github.com/kellyloria/Tahoe-streamflow-and-nearshore-metabolism.more » « less
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This dataset contains water quality measurements made on Green Lakes 1, 2, 3, 4, 5, and Lake Albion. Green Lake 4 was initially sampled in 2000 and is ongoing. Ongoing sampling of Green Lakes 1 was started in 2014 and ongoing sampling of Lake Albion was started in 2016. Water samples were collected for analysis of chlorophyll a and nutrient analysis (which is available in glvwatsolu.dm.data) and field measurements for pH, temperature, specific conductivity, dissolved oxygen (DO), % saturation, secchi depth, PAR. Secchi depth is recorded at the 0m row however it is a measurement of depth and so the units are meters. Most samples were collected between 0800 and 1200 MST. The first sampling date each summer occurs shortly after the ice had melted. Data are collected from an inflatable raft at the point of deepest depth or from the lake inlet and outlet when surface flow is present. The majority of chlorophyll-a the measurements were taken at the surface (0m), the metalimnion (3m), and the hypolimnion nine (usually 8-11m). However, additional measurements were taken for side projects of the long-term dataset during several of the years and are included in this dataset. Water samples from the metalimnion or hypolimnion were collected using a Van Dorne sampler, and surface samples were collected as grab samples from the water column surface, the inlet and outlet. Field measurements were conducted using a YSI either DO or multiple probe meter (2014-2017, YSI MPS 556)(2018-ongoing, YSI ProPlus) and a Li-Cor meter with a quantum sensor. Chlorophyll-a was extracted from filtered samples and absorbance was measured before and after acidification to quantify chlorophyll a concentration.more » « less
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High-resolution water quality data are fundamental to observing rapid ecological responses to meteorology, climate, and other disturbance events. Here we describe the deployment of a single buoy line with multiple sensors at fixed-depths from a subsurface float in the water-column of Green Lake 4 (GL4). Sensors on the buoy collect data in both summer and winter, thereby providing valuable insights into lake characteristics beyond our standard sampling period, including key transitional periods such as ice formation and ice break-up.more » « less
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High-resolution water quality data are fundamental to observing rapid ecological responses to meteorology, climate, and other disturbance events. Here we describe the deployment of a single buoy line with multiple sensors at fixed-depths from a subsurface float in the water-column of Green Lake 4 (GL4). Sensors on the buoy collect data in both summer and winter, thereby providing valuable insights into lake characteristics beyond our standard sampling period, including key transitional periods such as ice formation and ice break-up.more » « less
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High-resolution water quality data are fundamental to observing rapid ecological responses to meteorology, climate, and other disturbance events. Here we describe the deployment of a single buoy line with multiple sensors at fixed-depths from a subsurface float in the water-column of Green Lake 4 (GL4). Sensors on the buoy collect data in both summer and winter, thereby providing valuable insights into lake characteristics beyond our standard sampling period, including key transitional periods such as ice formation and ice break-up.more » « less
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High-resolution water quality data are fundamental to observing rapid ecological responses to meteorology, climate, and other disturbance events. Here we describe the deployment of a single buoy line with multiple sensors at fixed-depths from a subsurface float in the water-column of Green Lake 4 (GL4). Sensors on the buoy collect data in both summer and winter, thereby providing valuable insights into lake characteristics beyond our standard sampling period, including key transitional periods such as ice formation and ice break-up.more » « less
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Beisner, Beatrix E (Ed.)Abstract The prolonged ice cover inherent to alpine lakes incurs unique challenges for aquatic life, which are compounded by recent shifts in the timing and duration of ice cover. To understand the responses of alpine zooplankton, we analyzed a decade (2009–2019) of open-water samples of Daphnia pulicaria and Hesperodiaptomus shoshone for growth, reproduction and ultraviolet radiation tolerance. Due to reproductive differences between taxa, we expected clonal cladocerans to exhibit a more rapid response to ice-cover changes relative to copepods dependent on sexual reproduction. For D. pulicaria, biomass and melanization were lowest after ice clearance and increased through summer, whereas fecundity was highest shortly after ice-off. For H. shoshone, biomass and fecundity peaked later but were generally less variable through time. Among years, ice clearance date varied by 49 days; years with earlier ice-out and a longer growing season supported higher D. pulicaria biomass and clutch sizes along with greater H. shoshone fecundity. While these large-bodied, stress tolerant zooplankton taxa were relatively resilient to phenological shifts during the observation period, continued losses of ice cover may create unfavorably warm conditions and facilitate invasion by montane species, emphasizing the value of long-term data in assessing future changes to these sensitive ecosystems.more » « less
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